Journal: npj Imaging

Article Title: [3- 11 C]Pyruvate PET detects alterations in cardiac pyruvate metabolism induced by doxorubicin chemotherapy

doi: 10.1038/s44303-026-00165-8

Figure Lengend Snippet: a [3- 14 C]Pyruvate flux in HCMs was evaluated in a time-dependent manner (0, 2, 4, 6, and 8 min). Cells were treated with vehicle (control; CTRL) or 0.1 µM DOX for 2 × 48 h ( n = 6 per condition). b Western blot analysis of MPC1, MPC2, and MCT1 expression from HCM treated with vehicle (CTRL), UK5099 (100 µM for 72 h), or DOX (0.1 µM for 2 × 48 h). Vinculin was used as a reference. c [3- 14 C]Pyruvate uptake was assessed in HCMs treated with vehicle (CTRL), UK5099 (100 µM for 72 h), or DOX (0.1 µM for 2 × 48 h) for 8 min. d [3- 14 C]Pyruvate uptake in HCM treated with scramble siRNA (100 nM for 24 h) or MPC1/2 siRNA (50 nM of each MPC siRNA—total concentration of 100 nM—for 24 h) for 8 min. e Hypothesized effect of DOX treatment on the cellular fate of [3- 14 C]pyruvate, which can be retained within the cell as [3- 14 C]pyruvate or another labeled metabolite, released into the media as [3- 14 C]lactate, [3- 14 C]pyruvate, or another species, or released to the air as [ 14 C]CO 2 . The illustration was created with BioRender. f [ 14 C]CO₂ capture assay was performed following an 8-min incubation of HCM with [3- 14 C]pyruvate. Data are presented as the mean ± s.d. * p < 0.05; *** p < 0.001; **** p < 0.0001. Statistical analysis was performed using Pearson correlation coefficients ( a R 2 = 0.9728), two-way ANOVA ( c ), unpaired t-test ( d ), or one-way ANOVA ( f ). HCM human cardiomyocytes.

Article Snippet: Human cardiomyocytes (HCMs) isolated from adult left ventricles (PromoCell, Heidelberg, Germany) were cultured according to the manufacturer’s instructions.

Techniques: Control, Western Blot, Expressing, Concentration Assay, Labeling, Incubation